Nature Communications (Feb 2016)

Structural hot spots for the solubility of globular proteins

  • Ashok Ganesan,
  • Aleksandra Siekierska,
  • Jacinte Beerten,
  • Marijke Brams,
  • Joost Van Durme,
  • Greet De Baets,
  • Rob Van der Kant,
  • Rodrigo Gallardo,
  • Meine Ramakers,
  • Tobias Langenberg,
  • Hannah Wilkinson,
  • Frederik De Smet,
  • Chris Ulens,
  • Frederic Rousseau,
  • Joost Schymkowitz

DOI
https://doi.org/10.1038/ncomms10816
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 15

Abstract

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Mutations in aggregation prone regions of recombinant proteins often improve their solubility, although they might cause negative effects on their structure and function. Here, the authors identify proteins hot spots that can be exploited to optimize solubility without compromising stability.