Cellulosic fraction of rice bran fibre alters the conformation and inhibits the activity of porcine pancreatic lipase

Jing Qi; Yue Li; Wallace Yokoyama; Hamid Majeed; Kingsley George Masamba; Fang Zhong; Jianguo Ma

Journal of Functional Foods (Dec 2015)

Cellulosic fraction of rice bran fibre alters the conformation and inhibits the activity of porcine pancreatic lipase

Jing Qi,
Yue Li,
Wallace Yokoyama,
Hamid Majeed,
Kingsley George Masamba,
Fang Zhong,
Jianguo Ma

Affiliations

Jing Qi: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Yue Li: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Wallace Yokoyama: Western Regional Research Center, ARS, USDA, Albany, CA 94710, USA
Hamid Majeed: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Kingsley George Masamba: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Fang Zhong: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Corresponding author. Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Tel.: +86 510 85197876; fax: +86 510 85197876.
Jianguo Ma: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Journal volume & issue: Vol. 19
pp. 39 – 48

Abstract

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A cellulosic rice bran insoluble dietary fibre (RBIDF) with different structures and physicochemical properties is shown to inhibit the activity of pancreatic lipase (PL) at different levels. The adsorption rate and saturation level to PL were greatest for RBIDF-2.0 (modified with 2.0% H2SO4 + 1.25% KOH), which had the highest specific surface area and oil holding capacity. The conformational deformation of unbound PL in the supernatant of PL-RBIDF confirmed by circular dichroism (CD) suggested that not only bound but also unbound PL activities were altered. Free energy calculations using data from fluorescence spectroscopy revealed that binding of PL to fibre depends primarily on electrostatic interactions, and the binding process is spontaneous and exothermic. This study indicates that dietary intake of cellulosic fractions of rice bran may be useful in reducing the bioavailability of dietary fat by altering the conformation and activity of PL that might be useful to control obesity.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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