Detection and quantification of adenovirus, polyomavirus, and papillomavirus in urban sewage

Abstract

The objective of this study was to assess the occurrence and seasonal frequency of human adenovirus (HAdV), human polyomavirus (HPyV), and human papillomavirus (HPV) in urban sewage. The detection of these viruses was carried out by polymerase chain reaction (PCR), and then the viral concentrations in the positive samples were quantified by quantitative PCR (qPCR). Additionally, HAdV and HPyV genotyping was also performed by PCR. A total of 38/60 (63.3%) positive samples were found. HAdV was the most prevalent virus (26/60; 43.3%), followed by HPyV (21/60; 35%) and HPV (21/60; 35%). The viral concentrations ranged from 3.56 × 102 to 7.55 × 107 genome copies/L. The most common dual viral agents was found between HAdV and HPyV, in eight samples (8/38, 21%). HAdV types 40 and 41 as well as HPyV types JC and BK were identified, with HAdV-40 and HPyV JC being the most prevalent types. Furthermore, the detection rates of HAdV, HPyV, and HPV were higher during the winter season than the other seasons. The high prevalence of HAdV and HPyV supports their suitability as viral indicators of sewage contamination. Furthermore, this study demonstrates the advantages of environmental surveillance as a tool to elucidate the community-circulating viruses. HIGHLIGHTS This study aimed to investigate the occurrence and seasonality variation of HAdV, HPyV, and HPV in raw sewage collected regularly over 12 months from sewage pump stations.; This study represents the first data about the occurrence of HAdV, HPyV, and HPV in Zagazig city, Al Sharqia Governorate, Egypt.; This study supports using both HAdV and HPyV as indicators than either of them as a single fecal indicator.;

Keywords

• adenovirus
• papillomavirus
• polyomavirus
• qpcr
• sewage