INTERRELATION OF CYTOGENETIC ABERRATIONS AND EXPRESSION OF IMMUNOPHENOTYPIC MARKERS ON BONE MARROW BLAST CELLS IN PRIMARY MYELODISPLASTIC SYNDROMES IN ADULTS

Abstract

Background. Myelodisplastic syndromes (MDS) are a heterogeneous group of clonal hematological diseases and are not associated with specific chromosomal aberrations. The aim of the study is to identify the most significant immunophenotypic aberrations as criteria for the prognosis of the disease based on their relationship with the cytogenetic characteristics of blast cells of the bone marrow in patients with primary MDS. Material and methods. The study included 116 adult patients with MDS. The expression of antigens on bone marrow blast cells was determined by the method of eight-color flow cytofluorimetry. The study of the karyotype was performed using the standard GTG method. Results. The presence of aberrant expression of CD56 and a decrease in the expression of CD38 on blast cells have been found to be associated with the presence of prognostically significant chromosomal abnormalities -7 / del (7q-), -5 / del (5q-). It has been proved that aberrant expression of CD25, CD5, CD19 and a decrease or lack of CD34 expression on blast cells correlate with monosomy 8, 9, and 20 chromosomes involved in the monosomal karyotype. Conclusion. A statistical relationship between immunophenotypic aberrations and certain cytogenetic disorders with different prognostic significance was revealed.

Keywords

• myelodysplastic syndrome
• aberrant immunophenotype
• cytogenetic aberrations
• monosomal karyotype