STAR Protocols (Sep 2023)

Mapping neuronal ensembles and pattern-completion neurons through graphical models

  • Darik A. O’Neil,
  • Alejandro Akrouh,
  • Rafael Yuste

Journal volume & issue
Vol. 4, no. 3
p. 102543

Abstract

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Summary: Neuronal ensembles are coordinated groups of neurons that serve as functional building blocks of neural circuits. Here, we present PatMap, a computational toolbox for identifying pattern-completion neurons, key trigger cells capable of reactivating entire neuronal ensembles. We describe a protocol for modeling neural circuits as probabilistic graphical models, linking behavior with specific neuronal ensembles, and identifying their pattern-completion neurons. By linking the cellular and circuit level, PatMap provides a springboard for targeted manipulation and control of neural circuits.For complete details on the use and execution of this protocol, please refer to Carrillo-Reid et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

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