Nature Communications (Jul 2021)
Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through ‘reverse phenotyping’
- David S. Fischer,
- Meshal Ansari,
- Karolin I. Wagner,
- Sebastian Jarosch,
- Yiqi Huang,
- Christoph H. Mayr,
- Maximilian Strunz,
- Niklas J. Lang,
- Elvira D’Ippolito,
- Monika Hammel,
- Laura Mateyka,
- Simone Weber,
- Lisa S. Wolff,
- Klaus Witter,
- Isis E. Fernandez,
- Gabriela Leuschner,
- Katrin Milger,
- Marion Frankenberger,
- Lorenz Nowak,
- Katharina Heinig-Menhard,
- Ina Koch,
- Mircea G. Stoleriu,
- Anne Hilgendorff,
- Jürgen Behr,
- Andreas Pichlmair,
- Benjamin Schubert,
- Fabian J. Theis,
- Dirk H. Busch,
- Herbert B. Schiller,
- Kilian Schober
Affiliations
- David S. Fischer
- Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg
- Meshal Ansari
- Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg
- Karolin I. Wagner
- Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM)
- Sebastian Jarosch
- Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM)
- Yiqi Huang
- Institute of Virology, Technische Universität München (TUM)
- Christoph H. Mayr
- Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL)
- Maximilian Strunz
- Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL)
- Niklas J. Lang
- Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL)
- Elvira D’Ippolito
- Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM)
- Monika Hammel
- Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM)
- Laura Mateyka
- Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM)
- Simone Weber
- Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM)
- Lisa S. Wolff
- Institute of Virology, Technische Universität München (TUM)
- Klaus Witter
- Laboratory of Immunogenetics and Molecular Diagnostics, Department of Transfusion Medicine, Cell Therapeutic Agents and Hemostaseology, LMU Munich
- Isis E. Fernandez
- Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL)
- Gabriela Leuschner
- Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL)
- Katrin Milger
- Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL)
- Marion Frankenberger
- Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL)
- Lorenz Nowak
- Center for Thoracic Surgery Munich, Ludwig-Maximilians-University of Munich (LMU) and Asklepios Lung Clinic Munich-Gauting, Munich and Gauting
- Katharina Heinig-Menhard
- Center for Thoracic Surgery Munich, Ludwig-Maximilians-University of Munich (LMU) and Asklepios Lung Clinic Munich-Gauting, Munich and Gauting
- Ina Koch
- Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL)
- Mircea G. Stoleriu
- Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL)
- Anne Hilgendorff
- Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL)
- Jürgen Behr
- Department of Medicine V, University Hospital, LMU Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for lung research (DZL)
- Andreas Pichlmair
- Institute of Virology, Technische Universität München (TUM)
- Benjamin Schubert
- Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg
- Fabian J. Theis
- Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg
- Dirk H. Busch
- Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM)
- Herbert B. Schiller
- Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum Muenchen, Member of the German Center for Lung Research (DZL)
- Kilian Schober
- Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM)
- DOI
- https://doi.org/10.1038/s41467-021-24730-4
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 14
Abstract
High resolution characterisation of the virus specific T cell response to SARS CoV2 provides further understanding to the immune response to the infection. Here the authors apply a reverse phenotyping approach to interrogate the SARS-CoV-2 T cell compartment at single cell resolution.”
