Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows

Vinícius da Silva Duarte; Roberto Sousa Dias; Andrew M. Kropinski; Stefano Campanaro; Laura Treu; Carolina Siqueira; Marcella Silva Vieira; Isabela da Silva Paes; Gabriele Rocha Santana; Franciele Martins; Josicelli Souza Crispim; André da Silva Xavier; Camila Geovana Ferro; Pedro M. P. Vidigal; Cynthia Canêdo da Silva; Sérgio Oliveira de Paula

doi:10.1038/s41598-018-24896-w

Scientific Reports (May 2018)

Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows

Vinícius da Silva Duarte,
Roberto Sousa Dias,
Andrew M. Kropinski,
Stefano Campanaro,
Laura Treu,
Carolina Siqueira,
Marcella Silva Vieira,
Isabela da Silva Paes,
Gabriele Rocha Santana,
Franciele Martins,
Josicelli Souza Crispim,
André da Silva Xavier,
Camila Geovana Ferro,
Pedro M. P. Vidigal,
Cynthia Canêdo da Silva,
Sérgio Oliveira de Paula

Affiliations

Vinícius da Silva Duarte: Department of Microbiology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Roberto Sousa Dias: Department of Microbiology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Andrew M. Kropinski: Departments of Food Science, and Pathobiology, University of Guelph, Guelph
Stefano Campanaro: Department of Biology, University of Padova
Laura Treu: Department of Biology, University of Padova
Carolina Siqueira: Department of General Biology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Marcella Silva Vieira: Department of General Biology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Isabela da Silva Paes: Department of General Biology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Gabriele Rocha Santana: Department of General Biology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Franciele Martins: Department of General Biology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Josicelli Souza Crispim: Department of Microbiology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
André da Silva Xavier: Embrapa Maize and Sorghum, Rodovia MG 424
Camila Geovana Ferro: Department of Plant Patology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Pedro M. P. Vidigal: Núcleo de Análise de Biomoléculas (NuBioMol), Center of Biological Sciences, Federal University of Viçosa
Cynthia Canêdo da Silva: Department of Microbiology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário
Sérgio Oliveira de Paula: Department of General Biology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário

DOI: https://doi.org/10.1038/s41598-018-24896-w
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 12

Abstract

Read online

Abstract Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efficient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shfl2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-inflammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

About the journal