Regulatory Mechanisms in Biosystems (Jul 2023)
Genotype-mutagenic interaction in the cytogenetic variability of winter wheat for a new ecogenetic factor
Abstract
The study of cytogenetic parameters of the activity of new mutagenic factors aims to reliably establish the possibilities of these factors in terms of variability depending on the subject of mutagenic action, the optimal use of certain factor sizes, the combination of the selected protocol with optimization of the yield of mutant forms in the future. Seeds of eight varieties of winter wheat (Balaton, Borovytsia, Zeleny Gai, Zoloto Ukrainy, Kalancha, Niva Odeska, Polyanka, Pochayna) were treated by SA (sodium azide) at concentrations of 0.010%, 0.025%, 0.05%, 0.10%. They were soaked in water solution for 24 hours. Cytogenetic activity was evaluated by pollen sterility, evaluation of general rates and indicators of spectra of chromosomal abnormalities at medium phases of cell mitosis according to wheat variety and chemical agent concentrations. As a result of the study, the key importance of the genotype-mutagenic interaction was demonstrated within the limits of variability of the main indicators of the frequency and spectrum of chromosomal aberrations. It has been established that in the future it will be more optimal to use two varieties whose genotype-mutagenic specificity indicators are significantly higher and one should expect a more significant yield of promising mutant forms from them in the future, especially in combination with SA concentrations in the range of 0.025% and 0.05%. It is demonstrated that the main parameters that reflect genetically determined possibilities in susceptibility to the ecogenetic factor are pollen fertility, the overall frequency of chromosome aberrations, and the number of induced fragments. The use of other parameters displays the trend only partially or does not display it at all, as is the case with the use of rarer types of chromosomal rearrangements. The least promising forms have also been identified for use as starting material in treatments with this substance. It is demonstrated that, in general, this agent is characterized by the same patterns in the induction of cytogenetic activity as for other chemical supermutagens, with some variations depending on the starting material. In the future, it is planned to link the obtained data with the frequency and quality of the resulting hereditary changes, primarily complex biochemical and physiological ones, in order to improve the quality of plant products and various types of plant tolerance to adverse environmental conditions.
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