Annexin A7 and Its Related Protein Suppressor of Death Domains Regulates Migration and Proliferation of Hca-P Cells

Abstract

Objective: This study was to investigate whether annexin A7 (AnnexinA7, ANXA7) and its co-related protein tumor celldeath domain silencer [suppressor of death domains (SODD)] regulates the migratory phenotype of liver cancer cells.Materials and Methods: In this experimental study, expression of ANXA7 in Hca-P cells, PANXA7 downregulatedcells and PANXA7 unrelated sequence cells was detected by real-time quantitative polymerase chainreaction (PCR) at mRNA level and western blotting at protein level. Transwell migration and invasion assayswere performed to determine the migratory phenotype.Results: After inhibition of ANXA7 expression, expression of SODD protein was also significantly decreased (P<0.05).Transwell cell transfer experiments showed that number of tumor cells that penetrated into the cell membrane wassignificantly reduced after ANXA7 silencing (P<0.05). Transwell cell invasion assay showed that number of tumorcells penetrating into Matrigel was significantly reduced after ANXA7 down-regulation (P<0.05). The CCK8 assay wasmeasured at 0, 24 and 48 hours, and proliferation rate of PANXA7 lower weir cells was slower than that of Hca-P cellsand PANXA7 non-related sequence cells (P<0.05).Conclusion: SODD expression was decreased with the down-regulation of ANXA7. Down-regulating ANXA7 in Hca-Pcells decreased proliferation, migration and invasion of tumor cells.

Keywords

• anxa7
• lymph node metastasis
• transfection