Interleukin-8 level in the blood of rats with experimental osteoarthritis and hypothyroidism

Abstract

In the study, the level of interleukin-8 in the blood serum of rats with experimental model of osteoarthritis and hypothyroidism under the influence of NSAID s and paracetamol was estimated. The experiments were performed on 75 white nonlinear rats of both sexes. Experimental osteoarthritis was induced by single intra-articular administration of 0.1 ml of monoiodoacetic acid solution into the knee joint. Experimental hypothyroidism was reconstructed by enteral administration of 0.02% carbimazole solution, given with a drinking­ ration for 6 weeks. On the 42nd day of the experiment, the animals were divided into 14 groups and drug administration began daily for 5 days. The serum level of interleukin-8 was estimated on 42 and 47 days of the experiment by specific ELISA kits. A significant decrease (P ≤ 0.05) of the elevated cytokine level under the influence of pharmacotherapy was revealed. According to the degree of influence on degenerative-dystrophic process in the knee joint, the investigated drugs can be arranged as follows: diclofenac sodium > ibuprofen > nimesulide = meloxicam > celecoxib > paracetamol. The results obtained showed that the level of interleukin-8 could be used as an indicator of non-steroidal drugs and paracetamol anti-inflammatory activity under experimental osteoarthritis and hypothyroidism.

Keywords

• osteoarthritis
• hypothyroidism
• nsaids
• pharmacotherapy
• biochemical markers
• interleukin-8 (il 8)