Development and Validation of a Quantitative Analysis of Water-Soluble Vitamins Using High-Performance Thin-Layer Chromatography and Its Application to the Analysis of Nutraceuticals

Abstract

In this study, a high-performance thin-layer chromatography (HPTLC)-based method was developed for the quantification of five water-soluble vitamins, B2, B3, B6, B12, and C, using ethanol–water (70:30, v/v) as the mobile phase. The method was validated according to the International Conference on Harmonisation (ICH) guidelines and the limits of detection/limits of quantification were determined as 5.27/15.97 ng (Vitamin B2), 13.03/39.47 ng (Vitamin B3), 59.61/180.64 ng (Vitamin B6), 14.17/42.93 ng (Vitamin B12), and 119.27/361.42 ng (Vitamin C) per band, respectively. The usefulness of the validated method for quality control was demonstrated with the successful quantitative analysis of the five vitamins in a range of nutraceuticals.

Keywords

• high-performance thin-layer chromatography (HPTLC)
• water-soluble vitamins
• validation
• quantification
• multivitamins
• nutraceutical