Generating transcriptional regulatory networks from time-ordered stem cell differentiation RNA sequencing data

Abstract

Summary: We describe steps to 1) identify ascending and descending monotonic key genes from time-ordered stem cell differentiation expression data, 2) construct time-ordered transcriptional regulatory networks, and 3) infer the involvement of transcription factors along the differentiation process.For complete details on the use and execution of this protocol, please refer to Wong et al. (2020). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Bioinformatics
• Sequence analysis
• Genomics
• RNAseq
• Stem Cells
• Cell Differentiation