Acta Agrobotanica (Dec 2013)

Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants

  • B. Wojciechowska,
  • D. Czupryńska,
  • K. Nowak,
  • A. Ciepliński

DOI
https://doi.org/10.5586/aa.1983.007
Journal volume & issue
Vol. 36, no. 1-2
pp. 67 – 81

Abstract

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Hypocotyl fragments of Cucumis sativus L. were grown on two basic media: Linsmaier-Skoog's (1964) and White's (1943) modified by Street and McGregor (1952). These media were enriched by addition of some growth regulators (NAA, IAA, 2,4-D) in combination with kinetin. White's basic medium is not suitable for callus culture and for rhisogenesis of cucumber hypocotyls. Linsmaier and Skoog's medium was most suitable for growth of callus. After enrichment of the media with IAA, NAA and 2,4-D differences in the degree of taking root by the explants and callus formation were observed.