Influence of sample weight on amount of extracted protein from pelleted samples

Abstract

Protein is one of the major components of the pe1leted animal feed. Thus, it is very important to properly access protein quality and quantity. In this paper ground corn is pelleted on a flat die pellet press. The extraction buffer (0.125 M Tris-HCl at pH 6.8 containing 10% β-mercaptoethanol, 4% sodium dodecyl sulfate, 20% glycerol) was used for extraction of protein from pelleted samples. Additionally, Lab-on-a-chip (LoaC) electrophoresis is used for qualitative and quantitative characterization of protein from pelleted samples. Sample weights used for analysis were 30, 40 and 50 mg. Relative protein concentration (mass of protein in the sample per sample weight) was highest for sample weight of 30 mg, and it was 20,598.42 ppm, while concentrations of sample weights of 40 and 50 mg were 5,976.46 and 5,226.94 ppm, respectively. .

Keywords

• pelleting
• protein analysis
• loac electrophoresis