Učënye Zapiski Kazanskogo Universiteta. Seriâ Estestvennye Nauki (Dec 2016)
On the efficiency of introduction of microbial strains used for adjusted changes of the compost properties
Abstract
The use of suppressive composts, which exhibit fertilizing properties towards plants and inhibiting properties towards phytopathogens, instead of traditional pesticides is of growing practical and scientific importance. In this work, we have studied the possibility to obtain suppressive compost by introducing the microbial biopreparation and estimated the survival rate of the biopreparation microbial strains in the compost by means of the real-time PCR method. The biopreparation consisted of four microbial strains (Trichoderma asperellum T203, Pseudomonas putida PCL1760, Pseudomonas fluorescence WCS365, and Streptomyces spp.) characterized by four different mechanisms of plant pathogen inhibition. The compost was prepared using straw waste, chicken and cow manures. The compost has been inoculated with the biopreparation twice, on the 120th and 180th days of composting. Suppressiveness towards Fusarium oxysporum pathogen has been measured. Non-inoculated compost has been used as control. It has been found that single inoculation of the biopreparation increased suppressiveness by 1.4 times for up to 60 days. Repeated inoculation resulted in a more significant increase of suppressiveness with longer duration – by 8.5 times for up to 90 days. The peaks of suppressiveness coincided with the increased abundance of two of four biopreparation microbial strains – P. fluorescence WCS365 and T. asperellum T203.
