Species composition of microorganisms isolated from biomaterials of sick calves and cows

Abstract

Bacteriosis of cattle, the main cause of which is opportunistic microflora, cause significant damage to the livestock industry. Therefore, the study of the etiological structure of bacteriosis in cattle is relevant. During 2018, 2020–2021, 824 biomaterials’ samples of from 97 livestock farms in different regions of Ukraine were studied, including milk samples from patients with mastitis and vaginal lavages during cow endometritis, nasal mucus from calves with respiratory syndrome, feces from animals with diarrhea, exudate during inflammatory processes of the extremities. During bacteriological studies of biomaterials, 35 types of microorganisms were isolated. It should be noted that from 23.7% to 24.2% of Escherichia coli isolates were isolated; bacteria of the genus Proteus – from 10.8% to 16.7%. The important role of staphylococci in the occurrence of cattle bacteriosis has been proved, they have been isolated from 21.43% to 28.75%, while the proportion of Staphylococcus aureus isolation increased up to 21.6%. In the course of the biomaterials studies, up to 16.3% of enterococci were identified. According to our long-term research, simultaneous or sequential infection of animals with two or more pathogens has been established. Over the last year of observations (2021) the same trend was revealed towards an increase in the cases of two or more pathogens detection from one animals’ biomaterial with bacteriosis. If one is isolated in 31.11% of samples, while two – in 39.68%, three – in 18.41%.

Keywords

• bacteria
• isolate
• pathogen
• sample
• etiological structure