MOLECULAR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS FROM POULTRY IN THRISSUR DISTRICT

Abstract

Three ailing broilers of age 3 weeks, 5 weeks and 9 weeks respectively were presented to the Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy from three different private farms in Thrissur district for disease investigation. Owners reported a high mortality rate among the flocks and the birds were showing symptoms such as anorexia, depression, ruffled feathers, white watery diarrhoea and death. On post mortem examination, enlargement of bursa suggestive of infectious bursal disease (IBD) was noticed. The further confirmation of the cases were done with a reverse transcriptase polymerase chain reaction (RT-PCR) targeting the VP2 gene of Infectious bursal disease virus (IBDV), which revealed an amplicon size of 480 bp, specific for VP2 gene.

Keywords

• infectious bursal disease
• rt-pcr
• thrissur district