A simplified method for synthesizing juvenile hormone-protein conjugates.

Abstract

A convenient and simple method is presented for the synthesis of juvenile hormone-thyroglobulin conjugates. Methods previously described for this hapten linkage have used a two-step process in which a reactive intermediate was isolated and then used for conjugation. With the approach described here, an efficient, single-step conjugation reaction is effected between the carboxyl moiety of juvenile hormone III and the carrier protein, bovine thyroglobulin. Isolation of the reactive intermediate is eliminated. Isotopic dilution indicates that between 100 and 115 moles of hapten are conjugated per mole of carrier protein.