A mass spectrometric method for in-depth profiling of phosphoinositide regioisomers and their disease-associated regulation

Abstract

Different phosphoinositide isomers are involved in a variety of physiological and pathological processes. Here, the authors combine chiral column chromatography and mass spectrometry to measure phosphoinositide regioisomers, revealing their dynamic changes in intra- and extracellular cancer cell milieus.