First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

Tamara Alhamami; Piklu Roy Chowdhury; Nancy Gomes; Mandi Carr; Tania Veltman; Manouchehr Khazandi; Joanne Mollinger; Ania T. Deutscher; Conny Turni; Layla Mahdi; Henrietta Venter; Sam Abraham; Steven P. Djordjevic; Darren J. Trott

doi:10.3390/microorganisms9061322

Microorganisms (Jun 2021)

First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

Tamara Alhamami,
Piklu Roy Chowdhury,
Nancy Gomes,
Mandi Carr,
Tania Veltman,
Manouchehr Khazandi,
Joanne Mollinger,
Ania T. Deutscher,
Conny Turni,
Layla Mahdi,
Henrietta Venter,
Sam Abraham,
Steven P. Djordjevic,
Darren J. Trott

Affiliations

Tamara Alhamami: Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia
Piklu Roy Chowdhury: The ithree Institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, Australia
Nancy Gomes: Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia
Mandi Carr: Department of Animal Health and Production, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia
Tania Veltman: Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia
Manouchehr Khazandi: Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia
Joanne Mollinger: Biosecurity Sciences Laboratory, Department of Agriculture and Fisheries, Coopers Plains, QLD 4108, Australia
Ania T. Deutscher: NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568, Australia
Conny Turni: Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD 4072, Australia
Layla Mahdi: Clinical & Health Sciences, University of South Australia, Adelaide, SA 5000, Australia
Henrietta Venter: Clinical & Health Sciences, University of South Australia, Adelaide, SA 5000, Australia
Sam Abraham: Antimicrobial Resistance and Infectious Disease Laboratory, Murdoch University, Murdoch, WA 6150, Australia
Steven P. Djordjevic: The ithree Institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, Australia
Darren J. Trott: Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia

DOI: https://doi.org/10.3390/microorganisms9061322
Journal volume & issue: Vol. 9, no. 6
p. 1322

Abstract

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Bovine respiratory disease (BRD) causes high morbidity and mortality in beef cattle worldwide. Antimicrobial resistance (AMR) monitoring of BRD pathogens is critical to promote appropriate antimicrobial stewardship in veterinary medicine for optimal treatment and control. Here, the susceptibility of Mannheimia haemolytica and Pasteurella multicoda isolates obtained from BRD clinical cases (deep lung swabs at post-mortem) among feedlots in four Australian states (2014–2019) was determined for 19 antimicrobial agents. The M. haemolytica isolates were pan-susceptible to all tested agents apart from a single macrolide-resistant isolate (1/88; 1.1%) from New South Wales (NSW). Much higher frequencies of P. multocida isolates were resistant to tetracycline (18/140; 12.9%), tilmicosin (19/140; 13.6%), tulathromycin/gamithromycin (17/140; 12.1%), and ampicillin/penicillin (6/140; 4.6%). Five P. multocida isolates (3.6%), all obtained from NSW in 2019, exhibited dual resistance to macrolides and tetracycline, and a further two Queensland isolates from 2019 (1.4%) exhibited a multidrug-resistant phenotype to ampicillin/penicillin, tetracycline, and tilmicosin. Random-amplified polymorphic DNA (RAPD) typing identified a high degree of genetic homogeneity among the M. haemolytica isolates, whereas P. multocida isolates were more heterogeneous. Illumina whole genome sequencing identified the genes msr(E) and mph(E)encoding macrolide resistance, tet(R)-tet(H) or tet(Y) encoding tetracycline resistance, and blaROB-1 encoding ampicillin/penicillin resistance in all isolates exhibiting a corresponding resistant phenotype. The exception was the tilmicosin-resistant, tulathromycin/gamithromycin-susceptible phenotype identified in two Queensland isolates, the genetic basis of which could not be determined. These results confirm the first emergence of AMR in M. haemolytica and P. multocida from BRD cases in Australia, which should be closely monitored.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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