Transforming Growth Factor Β: Clinical Significance and Peculiarities of Inheritance in Children with Atopic Dermatitis

Abstract

Transforming growth factor β1 (TGF-β1) plays an important role in the pathogenesis of many allergic diseases, including atopic dermatitis (AD). Objective: to study the role of TGF-β1 in the pathogenesis of AD in children. Materials and methods. A survey was conducted of 34 patients with AD and 20 healthy children. TGF-β1 in serum were determined by ELISA using kits of Human TGF beta1 Platinum ELISA. Definition Arg25Pro gene polymorphism of TGF β1 was carried out using allele-specific polymerase chain reaction. Results. It was found that patients with AD showed a significant increase in the concentration of TGF-β in the serum (222,37±68,58 PG/ml) compared with the control group (3,03±0,41 ng/ml) [p = 0.001]. The concentration depends on the severity and duration of the illness. Frequency of genotypes for the polymorphism Arg25Pro TGF β1 gene among patients with AD had significant differences from the control group (p < 0.05). Moreover, among patients who are heterozygotes for the gene Arg25Pro TGF β1, was significantly more observed moderate (77.78 %) and severe (33,33%) the course of the disease. Conclusion. The obtained results confirm the importance of TGF-β1 in the pathogenesis of AD.

Keywords

• Atopic dermatitis
• Transforming growth factor β (TGF-β)
• Children
• Genetics
• Gene polymorphism
• Pathogenesis