Protein Sequence Retrieval and Phylogenetic Analysis of Various α-amylase Producing Bacillus species

Abstract

Due to advancements made in biotechnology, genetic manipulation and medium optimization has been possible and that has led to high yield in enzyme especially α-amylase with improvised properties that find its application suitably in detergent, laundry and starch processing industries. Various species of the genus Bacillus produce α amylases with varying properties. The amylases that find application miraculously in different fields can be easily obtained from plant, animal and microbial sources. Out of the three sources, microbial source especially the genus Bacillus is extensively studied for its ability to produce the enzyme. In the present study, protein sequences of primarily identified α-amylase producing Bacillus spp. were retrieved from protein database of National Center for Biotechnology Information. Multiple sequence alignment was performed and construction of Phylogenetic tree among these sequences was carried out using Neighbor-joining method; data sets based on 1000 re-samplings in molecular genetics evolutionary analysis.

Keywords

• alkaline α amylase
• bacillus
• phylogenetic analysis
• protein sequence