Frontiers in Marine Science (May 2022)
Massive Heat Shock Protein 70 Genes Expansion and Transcriptional Signatures Uncover Hard Clam Adaptations to Heat and Hypoxia
- Zhi Hu,
- Zhi Hu,
- Zhi Hu,
- Zhi Hu,
- Zhi Hu,
- Zhi Hu,
- Hao Song,
- Hao Song,
- Hao Song,
- Hao Song,
- Hao Song,
- Jie Feng,
- Jie Feng,
- Jie Feng,
- Jie Feng,
- Jie Feng,
- Cong Zhou,
- Cong Zhou,
- Cong Zhou,
- Cong Zhou,
- Cong Zhou,
- Cong Zhou,
- Mei-Jie Yang,
- Mei-Jie Yang,
- Mei-Jie Yang,
- Mei-Jie Yang,
- Mei-Jie Yang,
- Pu Shi,
- Pu Shi,
- Pu Shi,
- Pu Shi,
- Pu Shi,
- Pu Shi,
- Zheng-Lin Yu,
- Yong-Ren Li,
- Yong-Jun Guo,
- Hai-Zhou Li,
- Tao Zhang,
- Tao Zhang,
- Tao Zhang,
- Tao Zhang,
- Tao Zhang
Affiliations
- Zhi Hu
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Zhi Hu
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Zhi Hu
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Zhi Hu
- CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Zhi Hu
- University of Chinese Academy of Sciences, Beijing, China
- Zhi Hu
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Hao Song
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Hao Song
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Hao Song
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Hao Song
- CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Hao Song
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Jie Feng
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Jie Feng
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Jie Feng
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Jie Feng
- CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Jie Feng
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Cong Zhou
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Cong Zhou
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Cong Zhou
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Cong Zhou
- CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Cong Zhou
- University of Chinese Academy of Sciences, Beijing, China
- Cong Zhou
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Mei-Jie Yang
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Mei-Jie Yang
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Mei-Jie Yang
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Mei-Jie Yang
- CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Mei-Jie Yang
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Pu Shi
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Pu Shi
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Pu Shi
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Pu Shi
- CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Pu Shi
- University of Chinese Academy of Sciences, Beijing, China
- Pu Shi
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- Zheng-Lin Yu
- Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China
- Yong-Ren Li
- Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China
- Yong-Jun Guo
- Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China
- Hai-Zhou Li
- Shandong Fu Han Ocean Sci-Tech Co., Ltd, Haiyang, China
- Tao Zhang
- Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Tao Zhang
- Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
- Tao Zhang
- Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
- Tao Zhang
- CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
- Tao Zhang
- Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao, China
- DOI
- https://doi.org/10.3389/fmars.2022.898669
- Journal volume & issue
-
Vol. 9
Abstract
Heat shock protein 70 (HSP70) members participate in a wide range of housekeeping and stress-related activities in eukaryotic cells. In marine ecosystems, bivalves encounter abiotic stresses, including high temperatures and low dissolved oxygen. Here, 133 MmHSP70 genes were identified through combined methods including Blastp, HMM and manual filtration, based on the whole Mercenaria mercenaria genome. The MmHSP70 genes were unevenly distributed, and 41 genes (33.08%) were located on Chr 7. Phylogenetic analyses indicated that the MmHSP70 gene family mainly consisted of two clusters and the Hspa12 subfamily underwent lineage-specific expansion. A high-density collinear gene block was observed between M. mercenaria Chr 7 and Cyclina sinensis Chr 14. Tandem duplication MmHSP70 gene pairs experienced different levels of purifying selection, which could be an important source of sequence and functional constraints. MmHSP70 genes showed tissue-specific and stress-specific expression. Most tandem duplication HSP70 gene pairs had high expression under hypoxia stress. HSP70 B2 tandem duplication gene pairs showed significantly increased expression under heat plus severe hypoxia stress. This study provided a comprehensive understanding of the MmHSP70 gene family in the M. mercenaria and laid a significant foundation for further studies on the functional characteristics of MmHSP70 genes during exposure to heat and hypoxia stress.
Keywords