Ca2+-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair

Patrick Niekamp; Felix Scharte; Tolulope Sokoya; Laura Vittadello; Yeongho Kim; Yongqiang Deng; Elisabeth Südhoff; Angelika Hilderink; Mirco Imlau; Christopher J. Clarke; Michael Hensel; Christopher G. Burd; Joost C. M. Holthuis

doi:10.1038/s41467-022-29481-4

Nature Communications (Apr 2022)

Ca2+-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair

Patrick Niekamp,
Felix Scharte,
Tolulope Sokoya,
Laura Vittadello,
Yeongho Kim,
Yongqiang Deng,
Elisabeth Südhoff,
Angelika Hilderink,
Mirco Imlau,
Christopher J. Clarke,
Michael Hensel,
Christopher G. Burd,
Joost C. M. Holthuis

Affiliations

Patrick Niekamp: Molecular Cell Biology Division, Department of Biology and Center of Cellular Nanoanalytics, University of Osnabrück
Felix Scharte: Microbiology Division, Department of Biology and Center of Cellular Nanoanalytics, University of Osnabrück
Tolulope Sokoya: Molecular Cell Biology Division, Department of Biology and Center of Cellular Nanoanalytics, University of Osnabrück
Laura Vittadello: Experimental Physics Division, Department of Physics and Center of Cellular Nanoanalytics, University of Osnabrück
Yeongho Kim: Department of Cell Biology, Yale School of Medicine
Yongqiang Deng: Department of Cell Biology, Yale School of Medicine
Elisabeth Südhoff: Molecular Cell Biology Division, Department of Biology and Center of Cellular Nanoanalytics, University of Osnabrück
Angelika Hilderink: Molecular Cell Biology Division, Department of Biology and Center of Cellular Nanoanalytics, University of Osnabrück
Mirco Imlau: Experimental Physics Division, Department of Physics and Center of Cellular Nanoanalytics, University of Osnabrück
Christopher J. Clarke: Department of Medicine and Cancer Center, Stony Brook University
Michael Hensel: Microbiology Division, Department of Biology and Center of Cellular Nanoanalytics, University of Osnabrück
Christopher G. Burd: Department of Cell Biology, Yale School of Medicine
Joost C. M. Holthuis: Molecular Cell Biology Division, Department of Biology and Center of Cellular Nanoanalytics, University of Osnabrück

DOI: https://doi.org/10.1038/s41467-022-29481-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

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Activation of ESCRT prevents potentially lethal outcomes of minor perturbations in lysosomal integrity. Here authors show that Ca2 + -activated scrambling of sphingomyelin and its cytosolic turnover drives lysosomal repair independently of ESCRT.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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