PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's Arteritis

Abstract

Objectives This study aims to investigate whether PD-1 expressions are abnormal in patients with TAK. Methods PD-1 expression was analyzed by flow cytometry. Serum cytokines IL-10, IL-7, IL-2, IL-15, CCL2, CCL3, and CXCL10 were detected using a cytokine cytometric bead array. Immunohistochemistry staining analysis was used to test PD-1 and programmed death-ligand 1 (PD-L1) expression in the aorta of three patients with TAK and three patients with atherosclerosis as controls. Results The mean fluorescence intensity of PD-1 in CD4 + PD-1 + cells was decreased in patients with TAK and the frequency of CD4 + Foxp3 − PD-1 + cells among CD4 + T cells was also decreased in peripheral blood relative to healthy controls ( P .05). Patients with TAK had higher serum levels of IL-10, IL-7, CCL2, and CCL3 ( P < .05). Conclusions Abnormal expression of PD-1 in serum and aorta tissue of patients with TAK may contribute to TAK pathogenesis. Key points PD-1 expression in both peripheral blood and aorta tissue of TAK patients decreased relative to healthy controls, indicating that PD-1 might be involved in TAK pathogenesis.