Pesquisa Agropecuária Brasileira (Dec 2015)

Optimization of heterologous microsatellites in Piracanjuba

  • Fausto Moreira da Silva Carmo,
  • Érico Macedo Polo,
  • Marcos Antônio da Silva,
  • Gabriel de Menezes Yazbeck

DOI
https://doi.org/10.1590/S0100-204X2015001200015
Journal volume & issue
Vol. 50, no. 12
pp. 1236 – 1239

Abstract

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Abstract: The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba.

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