PLoS ONE (Jan 2023)

The microbiome of an outpatient rehabilitation clinic and predictors of contamination: A pilot study.

  • Gabriella Brigando,
  • Casey Sutton,
  • Olivia Uebelhor,
  • Nicholas Pitsoulakis,
  • Matthew Pytynia,
  • Thomas Dillon,
  • Teresa Elliott-Burke,
  • Nathaniel Hubert,
  • Kristina Martinez-Guryn,
  • Charlotte Bolch,
  • Mae J Ciancio,
  • Christian C Evans

DOI
https://doi.org/10.1371/journal.pone.0281299
Journal volume & issue
Vol. 18, no. 5
p. e0281299

Abstract

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BackgroundUnderstanding sources of microbial contamination in outpatient rehabilitation (REHAB) clinics is important to patients and healthcare providers.PurposeThe purpose of this study was to characterize the microbiome of an outpatient REHAB clinic and examine relationships between clinic factors and contamination.MethodsForty commonly contacted surfaces in an outpatient REHAB clinic were observed for frequency of contact and swiped using environmental sample collection kits. Surfaces were categorized based on frequency of contact and cleaning and surface type. Total bacterial and fungal load was assessed using primer sets specific for the 16S rRNA and ITS genes, respectively. Bacterial samples were sequenced using the Illumina system and analyzed using Illumina-utils, Minimum Entropy Decomposition, QIIME2 (for alpha and beta diversity), LEfSe and ANCOM-BC for taxonomic differential abundance and ADONIS to test for differences in beta diversity (pResultsPorous surfaces had more bacterial DNA compared to non-porous surfaces (median non-porous = 0.0016ng/μL, 95%CI = 0.0077-0.00024ng/μL, N = 15; porous = 0.0084 ng/μL, 95%CI = 0.0046-0.019 ng/μL, N = 18. p = 0.0066,DNA. Samples clustered by type of surface with non-porous surfaces further differentiated by those contacted by hand versus foot. ADONIS two-way ANOVA showed that the interaction of porosity and contact frequency (but neither alone) had a significant effect on 16S communities (F = 1.7234, R2 = 0.0609, p = 0.032).DiscussionPorosity of surfaces and the way they are contacted may play an underestimated, but important role in microbial contamination. Additional research involving a broader range of clinics is required to confirm results. Results suggest that surface and contact-specific cleaning and hygiene measures may be needed for optimal sanitization in outpatient REHAB clinics.