High transfection efficiency of neural stem cells with magnetofection

Abstract

Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.

Keywords

• Magnetofection
• transfection
• neural stem cells
• NSC
• NeuroMag
• neuronal differentiation