Frontiers of Agricultural Science and Engineering (Mar 2015)

Novel polymorphic EST-based microsatellite marker isolation and characterization from Poncirus trifoliata (Rutaceae)

  • Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG

DOI
https://doi.org/10.15302/J-FASE-2015048
Journal volume & issue
Vol. 2, no. 1
pp. 60 – 65

Abstract

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Novel Poncirus trifoliata simple sequence repeat (SSR) markers were developed to evaluate their utility for genetic diversity and breeding studies of P. trifoliata and related species. A total of 108 primer pairs were characterized by PCR amplification experiments. Among these, 61 were polymorphic and transferable to other citrus species. The number of alleles per locus ranged from 2 to 6, with an average of 2.37 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.83 and 0 to 1.00, respectively. These novel polymorphic SSR markers will be useful for citrus cultivar identification and evaluation as well as breeding studies.

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