HPLC Determination of Atrazine in Water Using Dispersive Liquid-Liquid Microextraction Based on Ultrasonic-assisted in situ Ionic Liquid Formation

Abstract

A kind of green solvent, ionic liquid, was introduced as an extracting agent, and a new method of dispersive liquid-liquid microextraction based on ultrasonic-assisted in situ ionic liquid formation (DLLM-UAISLF) was established for the High Performance Liquid Chromatography (HPLC) determination of atrazine in water. Five mL of water was treated using 80 μL of [HMim]Cl as the extracting agent, 400 μL of LiNTf2 as the ion-exchange material, ultrasound time of 10 minutes and centrifuge time of 8 minutes at 4000 r/min. During this process, the droplets of ionic liquid [HMim]NTf2 were formed in water and the precipitates were then separated for determination of atrazine by HPLC. The detection limit of atrazine is 0.01 mg/L and the detection values range from 0.01 to 0.5 mg/L. The proposed method yielded recoveries of 100.4%-106.7%, which are obviously better than those ( < 67%) of direct liquid-liquid microextraction. This method has potential in the determination of organic pollutants.

Keywords

• atrazine
• in situ ionic liquid formation
• dispersive liquid-liquid microextraction
• high performance liquid chromatography