Effects of human thermal transient receptor channel 1 gene transfection on cultured rabbit corneal endothelial cells <i>in vitro</i>

Abstract

AIM:To explore the effects of human thermal transient receptor channel 1 gene transfection on corneal endothelial cell of rabbits. METHODS:Research group were dealt for thermal transient receptor channel 1 gene mediated by liposome transfection to rabbit corneal endothelial cells. MTT method was used to observe its influence on cell proliferation. Immunohistochemical staining and computer image analysis system were used to test the effects for proliferation cell nucleus antigen(PCNA)expression. RESULTS:Proliferation of corneal endothelial cell of rabbit was promoted after thermal transient receptor channel 1 gene transfected and the difference between experiment group and control group(t=3.01,P=0.013). The expression of PCNA promoted after thermal transient receptor channel 1 gene transfected(t=3.21,P=0.007)compared with control group. CONCLUSION:The expression of PCNA in rabbit corneal endothelial cells can promote the proliferation of corneal endothelial cells of rabbits.

Keywords

• thermal transient receptor channel 1
• proliferating cell nuclear antigen
• corneal endothelial cell