Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population

Wenxin Zhang; Qiuting Dai; Yishi Hua; Weihao Di; Jinghui Guo; Jian Zhao; Yulei Deng; Ying Wang

Stem Cell Research (Mar 2021)

Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population

Wenxin Zhang,
Qiuting Dai,
Yishi Hua,
Weihao Di,
Jinghui Guo,
Jian Zhao,
Yulei Deng,
Ying Wang

Affiliations

Wenxin Zhang: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Qiuting Dai: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Yishi Hua: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China
Weihao Di: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Jinghui Guo: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Jian Zhao: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Yulei Deng: Neurology Department, Shanghai Ruijin Hospital, Jiaotong University School of Medicine, Shanghai, China; Corresponding authors.
Ying Wang: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Corresponding authors.

Journal volume & issue: Vol. 51
p. 102172

Abstract

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A 66-year-old Chinese Han Alzheimer's Disease (AD) female patient and her two unaffected children donated their Peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs. Pluripotency was confirmed by the ability of iPSCs to spontaneously differentiate three germ layers in vitro as well. The iPSC line displayed a normal karyotype. This model provides insight into further pathological studies to research identify early biomarkers, study disease pedigrees, and also for drug testing purposes.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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