Nanoscopic subcellular imaging enabled by ion beam tomography

Ahmet F. Coskun; Guojun Han; Shambavi Ganesh; Shih-Yu Chen; Xavier Rovira Clavé; Stefan Harmsen; Sizun Jiang; Christian M. Schürch; Yunhao Bai; Chuck Hitzman; Garry P. Nolan

doi:10.1038/s41467-020-20753-5

Nature Communications (Feb 2021)

Nanoscopic subcellular imaging enabled by ion beam tomography

Ahmet F. Coskun,
Guojun Han,
Shambavi Ganesh,
Shih-Yu Chen,
Xavier Rovira Clavé,
Stefan Harmsen,
Sizun Jiang,
Christian M. Schürch,
Yunhao Bai,
Chuck Hitzman,
Garry P. Nolan

Affiliations

Ahmet F. Coskun: Baxter Laboratory, Department of Microbiology and Immunology, Stanford University School of Medicine
Guojun Han: Baxter Laboratory, Department of Microbiology and Immunology, Stanford University School of Medicine
Shambavi Ganesh: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Shih-Yu Chen: Baxter Laboratory, Department of Microbiology and Immunology, Stanford University School of Medicine
Xavier Rovira Clavé: Baxter Laboratory, Department of Microbiology and Immunology, Stanford University School of Medicine
Stefan Harmsen: Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine
Sizun Jiang: Baxter Laboratory, Department of Microbiology and Immunology, Stanford University School of Medicine
Christian M. Schürch: Baxter Laboratory, Department of Microbiology and Immunology, Stanford University School of Medicine
Yunhao Bai: Department of Chemistry, Stanford University
Chuck Hitzman: Department of Materials Science and Engineering, Stanford University
Garry P. Nolan: Baxter Laboratory, Department of Microbiology and Immunology, Stanford University School of Medicine

DOI: https://doi.org/10.1038/s41467-020-20753-5
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 19

Abstract

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Secondary ion beam mass spectrometry (SIMS) is a method to obtain a chemical snapshot of biological tissue, but the spatial resolution is low. Here, the authors develop a computational and technology pipeline to localise a chemical signal in SIMS in 3D and sub-25 nm accuracy, called Ion Beam Tomography

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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