Effects of resveratrol, grape juice or red wine consumption Irisin levels and fibronectin type III domain containing protein 5 and uncoupoling protein gene expression modulation in rats

Abstract

Objectives: The aim was investigate resveratrol, grape juice, or red wine effects on FNDC5 and UCP2 gene expression on muscle and adipose tissue of Wistar rats receiving a high-fat diet. Methods: Five groups of female Wistar rats formed with five animals in each (Control Group-CG, High-Fat Group-HFG-20%, Grape Juice Group-GJG; Red Wine Group-RWG and Resveratrol Group-RG) were treated for 2 months. At the end, adipose and muscle tissues were collected, FNDC5 and UCP2 mRNA expressions were obtained by using real-time polymerase chain reaction. Irisin levels were assessed through ELISA. For comparison: two way ANOVA, using Duncan as post-test. Results: FNDC5 mRNA expression was increased in RG and GJG muscle (p < 0.001) and reduced in adipose tissue of all experimental groups (p < 0.001). The UCP2 mRNA expression was increased in muscle of HFG, RG and RWG (p < 0.01), compared with CG. Furthermore, increased expression of UCP2 mRNA in GJG adipose tissue (p < 0.05) was also observed when compared with the control group (CG). No significant differences were found concerning plasma irisin level in all animal groups. Conclusion: Resveratrol and grape juice were able to increase muscle tissue FNDC5 gene expression, and high-fat diet, red wine and resveratrol, increased UCP2 gene expression in this tissue. Grape juice was capable of increasing adipose tissue UCP2 gene expression. High-fat diet, isolated or associated to beverages rich in polyphenols, have decreased FNDC5 gene expression in adipose tissue. Nevertheless, the interventions did not affect irisin levels.

Keywords

• Irisin
• Resveratrol
• Grape juice
• Red wine
• Gene expression
• Muscle tissue