Effects of astaxanthin in diet on antioxidant capacity and molting-related genes expression of larvae giant freshwater prawn (Macrobrachium rosenbergii)

Junjie Xu; Xinyi Peng; Fangxi Li; ShaSha Zhang; Tao Wang; Xiaojing Dong; Longsheng Sun; Kaizhou Xie

Aquaculture Reports (Apr 2024)

Effects of astaxanthin in diet on antioxidant capacity and molting-related genes expression of larvae giant freshwater prawn (Macrobrachium rosenbergii)

Junjie Xu,
Xinyi Peng,
Fangxi Li,
ShaSha Zhang,
Tao Wang,
Xiaojing Dong,
Longsheng Sun,
Kaizhou Xie

Affiliations

Junjie Xu: Laboratory of Aquaculture Nutrition and Feed, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xinyi Peng: Laboratory of Aquaculture Nutrition and Feed, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Fangxi Li: Laboratory of Aquaculture Nutrition and Feed, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
ShaSha Zhang: Laboratory of Aquaculture Nutrition and Feed, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Tao Wang: Laboratory of Aquaculture Nutrition and Feed, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xiaojing Dong: Laboratory of Aquaculture Nutrition and Feed, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Longsheng Sun: Laboratory of Aquaculture Nutrition and Feed, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Corresponding author at: Laboratory of Aquaculture Nutrition and Feed, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China.
Kaizhou Xie: Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Corresponding author at: Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China.

Journal volume & issue: Vol. 35
p. 101956

Abstract

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This experiment was carried out to determine the effects of astaxanthin on reactive oxide species (ROS), antioxidant capacity as well as molting-related genes expression in larvae of giant freshwater prawn, Macrobrachium rosenbergii. A total of 90,000 larvae were assigned to five treatments with six replicates each. Astaxanthin mixed in steamed eggs with graded effective content as 0 (control, A0), 40 (A40), 80 (A80), 160 (A160) and 320 (A320) mg kg−1 were fed to larvae for 14 days. The results showed that ROS of larvae in astaxanthin treatments was significantly lower than the control (P < 0.05). T-AOC, T-SOD and GR activities under astaxanthin treatments generally showed a downward trend and decreased significantly than the control (P < 0.05). No significant difference was observed towards CAT activities among A40 and A80, but the values were markedly greater than A0, A160, A320 (P < 0.05). Larvae in A80 exhibited stronger MDA content than the other (P < 0.05). GSH-Px activity was significantly highest in A320 than the other treatments (P < 0.05). The expression of molt-inhibiting hormone (MIH) in A40 and A80 enhanced markedly than the control (P < 0.05). The elevated expression of Ecdysone receptor (EcR) was recorded only in A160 and A320 (P < 0.05). Chitinase (CHIT) expression among treatments was significantly downregulated than the control except for A40. Heat shock protein (HSP21) and chitooligosaccharidolytic beta-N-acetylglucosaminidase-like expression increased dramatically in A160 and A320 (P < 0.05). For actin 6 gene, higher expression was happened only in A40 compared to control, others presented drop expression in very low level (P < 0.05). This study provides a theoretical basis that 320 mg/kg astaxanthin was excessive level, and might decreased partial antioxidant indexes and influenced molting genes expression of larvae M. rosenbergii.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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