Metabolic profiling of synovial fluid in human temporomandibular joint osteoarthritis

Dahe Zhang; Dahe Zhang; Dahe Zhang; Dahe Zhang; Dahe Zhang; Dahe Zhang; Dahe Zhang; Yuxin Zhang; Yuxin Zhang; Yuxin Zhang; Yuxin Zhang; Yuxin Zhang; Yuxin Zhang; Yuxin Zhang; Simo Xia; Simo Xia; Simo Xia; Simo Xia; Simo Xia; Simo Xia; Simo Xia; Pei Shen; Pei Shen; Pei Shen; Pei Shen; Pei Shen; Pei Shen; Pei Shen; Chi Yang; Chi Yang; Chi Yang; Chi Yang; Chi Yang; Chi Yang; Chi Yang

doi:10.3389/fimmu.2024.1335181

Frontiers in Immunology (Mar 2024)

Metabolic profiling of synovial fluid in human temporomandibular joint osteoarthritis

Dahe Zhang,
Dahe Zhang,
Dahe Zhang,
Dahe Zhang,
Dahe Zhang,
Dahe Zhang,
Dahe Zhang,
Yuxin Zhang,
Yuxin Zhang,
Yuxin Zhang,
Yuxin Zhang,
Yuxin Zhang,
Yuxin Zhang,
Yuxin Zhang,
Simo Xia,
Simo Xia,
Simo Xia,
Simo Xia,
Simo Xia,
Simo Xia,
Simo Xia,
Pei Shen,
Pei Shen,
Pei Shen,
Pei Shen,
Pei Shen,
Pei Shen,
Pei Shen,
Chi Yang,
Chi Yang,
Chi Yang,
Chi Yang,
Chi Yang,
Chi Yang,
Chi Yang

Affiliations

Dahe Zhang: Department of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Dahe Zhang: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Dahe Zhang: National Center for Stomatology, Shanghai, China
Dahe Zhang: National Clinical Research Center for Oral Diseases, Shanghai, China
Dahe Zhang: Shanghai Key Laboratory of Stomatology, Shanghai, China
Dahe Zhang: Shanghai Research Institute of Stomatology, Shanghai, China
Dahe Zhang: Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China
Yuxin Zhang: Department of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Yuxin Zhang: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Yuxin Zhang: National Center for Stomatology, Shanghai, China
Yuxin Zhang: National Clinical Research Center for Oral Diseases, Shanghai, China
Yuxin Zhang: Shanghai Key Laboratory of Stomatology, Shanghai, China
Yuxin Zhang: Shanghai Research Institute of Stomatology, Shanghai, China
Yuxin Zhang: Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China
Simo Xia: Department of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Simo Xia: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Simo Xia: National Center for Stomatology, Shanghai, China
Simo Xia: National Clinical Research Center for Oral Diseases, Shanghai, China
Simo Xia: Shanghai Key Laboratory of Stomatology, Shanghai, China
Simo Xia: Shanghai Research Institute of Stomatology, Shanghai, China
Simo Xia: Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China
Pei Shen: Department of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Pei Shen: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Pei Shen: National Center for Stomatology, Shanghai, China
Pei Shen: National Clinical Research Center for Oral Diseases, Shanghai, China
Pei Shen: Shanghai Key Laboratory of Stomatology, Shanghai, China
Pei Shen: Shanghai Research Institute of Stomatology, Shanghai, China
Pei Shen: Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China
Chi Yang: Department of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Chi Yang: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Chi Yang: National Center for Stomatology, Shanghai, China
Chi Yang: National Clinical Research Center for Oral Diseases, Shanghai, China
Chi Yang: Shanghai Key Laboratory of Stomatology, Shanghai, China
Chi Yang: Shanghai Research Institute of Stomatology, Shanghai, China
Chi Yang: Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China

DOI: https://doi.org/10.3389/fimmu.2024.1335181
Journal volume & issue: Vol. 15

Abstract

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IntroductionTemporomandibular joint (TMJ) osteoarthritis (OA) is a common TMJ degenerative disease with an unclear mechanism. Synovial fluid (SF), an important component of TMJ, contains various proteins and metabolites that may directly contribute to OA. The present study aimed to investigate the influence of SF in TMJOA at the metabolite level.MethodsUntargeted and widely targeted metabolic profiling were employed to identify metabolic changes in SF of 90 patients with different TMJOA grades according to TMJ magnetic resonance imaging.ResultsA total 1498 metabolites were detected. Most of the metabolites were amino acids and associated metabolites, benzene and substituted derivatives, and lipids. Among patients with mild, moderate and severe TMJOA, 164 gradually increasing and 176 gradually decreasing metabolites were identified, indicating that biosynthesis of cofactors, choline metabolism, mineral absorption and selenocompound metabolism are closely related to TMJOA grade. Combined metabolomics and clinical examination revealed 37 upregulated metabolites and 16 downregulated metabolites in patients with pain, of which 19 and 26 metabolites were positively and negatively correlated, respectively, with maximum interincisal opening. A model was constructed to diagnose TMJOA grade and nine biomarkers were identified. The identified metabolites are key to exploring the mechanism of TMJOA.DiscussionIn the present study, a metabolic profile was constructed and assessed using a much larger number of human SF samples from patients with TMJOA, and a model was established to contribute to the diagnosis of TMJOA grade. The findings expand our knowledge of metabolites in human SF of TMJOA patients, and provide an important basis for further research on the pathogenesis and treatment of TMJOA.

Published in Frontiers in Immunology

ISSN: 1664-3224 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://journal.frontiersin.org/journal/immunology

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