Effect of Moderate Intensity Exercise on Body Weight and Blood Estrogen Level Ovariectomized Mice

Abstract

Estrogen is a hormone which is related to lipid oxidation, lypogenesis inhibition, and lipolysis rate. The purpose of this study was to determine the effect of moderate intensity exercise on body weight and blood estrogen level in ovarectomized mice (ovx). The study was held in the Laboratory of Biochemistry, Faculty of Medicine Universitas Airlangga during the period of April to May 2017 on old nineteen 3-4 months old female mice, , 20-30 grams of weight, which were divided into two groups: OVX (n=9, ovx without exercise) and OVX+E (n=10, ovx and moderate intensity exercise). Moderate intensity exercise in this study included swimming, three times a week at 3 pm for four weeks using a 6% of body weight load. Experiment data were analyzed by paired t-test and independent t-test using SPSS. The average body weights of the OVX group were 26.44 ± 1.94 grams and 29.89 ± 2.62 gram at pretest and posttest, respectively., while for the OVX+E group, the weights were 26.50 ± 2.59 grams and 26.40 ± 2.07 grams. There were asignificant intergroup body weight differences (∆OVX= 3.44 ± 2.55 gram and ∆OVX+E= -1.00 ± 1.37 gram) with p=0.004. Blood estrogen level in OVX+E (12.04 ±10.13 ng/ml) was higher than in control (OVX= 11.24 ± 8.33 ng/ml); however, the difference was not significant (p=0.855). Moderate intensity exercise reduces body weight, without any significant increase in blood estrogen level. Hence, the body weight loss is not caused by estrogen, which may be due to the short period of exercise in this study that the estrogen has not had the time to increase.

Keywords

• Moderate intensity exercise
• Ovariectomy
• Obesity
• Blood estrogen level