Zhongguo youzhi (Feb 2023)

红棕榈油的短期毒性试验研究

  • 李寒玲1，陆慧媛1，沈伟健1，杨峻豪2，牛跃庭2，苏志同1， 厉蓉蓉1，赵晓娜1，龙云凤1，周萍1，王毅谦1 LI Hanling1, LU Huiyuan1, SHEN Weijian1, YOONG Junhao2 , NIU Yueting2, SU Zhitong1, LI Rongrong1,ZHAO Xiaona1, LONG Yunfeng1, ZHOU Ping1, WANG Yiqian1

DOI
https://doi.org/10.19902/j.cnki.zgyz.1003-7969.210744
Journal volume & issue
Vol. 48, no. 2
pp. 58 – 64

Abstract

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为评价红棕榈油作为烹调用油的安全性，按照GB 15193.22—2014《食品安全国家标准28天经口毒性试验》对马来西亚产红棕榈油进行短期毒性试验。将100只SD大鼠分为5组，按照人体摄入量的5.12、10.24、20.48倍设置红棕榈油低、中、高剂量组，同时设置溶剂对照组（玉米油组）和阴性对照组（棕榈油组）。采用掺入饲料和经口灌胃两种方式同时连续给予大鼠红棕榈油28 d，每天观察大鼠临床表现，每周记录体质量、摄食量，计算食物利用率等指标，试验前和结束时对大鼠进行眼部检查，结束时进行血液学、血生化、尿常规检测，并进行大体解剖，计算脏器系数并进行组织病理学检查。结果表明，红棕榈油以雌性2.04、4.11、8.31 g/kg，雄性2.12、4.23、8.45 g/kg的实际剂量给予大鼠28 d，受试大鼠临床表现良好，体质量、食物利用率、脏器质量、脏器系数均无异常改变；血液学、血生化及尿常规指标均在正常范围；各脏器病理组织学检查均未见与受试样品有关的病理改变。短期毒性试验结果表明红棕榈油未对大鼠产生毒性效应，短期经口食用是安全的。 In order to evaluate the safety of red palm oil as cooking oil, the short-term toxicity test of red palm oil from Malaysia was conducted by GB 15193.22-2014 National food safety standard 28-day oral toxicity test. A total of 100 SD rats were devided into 5 groups, and the high, medium, low dose groups of red palm oil were set according to 20.48, 10.24, 5.12 times of human intake. Solvent control group (corn oil group) and negative control group (palm oil group) were also set. The rats were given both adulterated feed and oral gavage for 28 d. The clinical performance of the rats was observed daily, body weight and food intake were recorded weekly, and food utilization was calculated. Before and at the end of the test, rats′ eyes were examed. At the end of the test, the tests of hematology, blood biochemistry, routine urine, gross anatomy and histopathological examination were carried out on the main organs, and the organ coefficient was calculated. The results showed that giving red palm oil at the real dose of female 2.04, 4.11 g/kg and 8.31 g/kg and male rats 2.12, 4.23 g/kg and 8.45 g/kg for 28 d, the experimental rats were in good clinical performance, with no abnormal changes in body weight, food utilization, organ weight and organ coefficient. The results of hematology and biochemistry and routine urine indexes were within the normal range. The histopathological examination of each organ showed no pathological changes related to the tested sample.The red palm oil has no harmful effects on rat, and is safe for orally intake in a short period of time.

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