Identification, development, and application of cross-species intron-spanning markers in lentil (Lens culinaris Medik.)

Abstract

Lentil (Lens culinaris Medik) is one of the most important food legumes in the world. The use in lentil of molecular marker-assisted breeding is limited, owing to the low availability of polymorphic markers. In the present study, we developed a set of polymorphic intron-spanning markers (ISMs) using a cross-species mapping approach. In this approach, putative unique transcripts (PUTs) of L. culinaris were mapped onto the Medicago truncatula genome, exploiting its closeness with the lentil genome. Spliced alignment of the PUTs resulted in a total of 25,717 alignments, allowing the development of 1703 ISMs. From these, a subset of 105 ISMs were synthesized and validated with a 51% amplification success rate in 32 lentil genotypes. Of these ISMs, 40 (74%) were polymorphic and generated 2–11 alleles per locus in a genetically diverse panel of 32 lentil genotypes including wild species. This set of polymorphic ISMs along with their functional annotation data will be useful in lentil breeding. Keywords: Allele diversity, Cross-species, Genotypes, Introns, Lentil, Molecular markers