بوم‌شناسی آبزیان (Apr 2013)

Study of optimal conditions in order to the use of the cyanobacteria Synechococcus sp. ISC106 as a candidate for biodiesel production

  • Elahe Kiaei,
  • Neda Soltani,
  • Mahnaz Mazaheri Assadi,
  • Ramazanali Khavarinegad,
  • Mahruz Dezfulian

Journal volume & issue
Vol. 2, no. 4
pp. 51 – 40

Abstract

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Global warming due to fossil fuel, price increases, environmental pollution resulting from their use and production green house gases caused scientists trying to produce proper fuel, renewable of replacing fossil fuels. The purpose of this study was to determine the conditions of survival, growth, pigments and fatty acid profiles to biodiesel production from cyanobacteria Synechococcus ISC 106 in nitrogen and pH different levels. Sampling was done of Kharg Island. After purification, the samples were cultured in BG11 at temperature 25ºC under a light and pH 7 were kept. Examples were identified of semi- permanent slides and molecular methods. Then the samples were treated under concentrations of 0.25, 0.5, 1 and 1.5 (g/L) NaNO3 and pH 5, 7, 9 growth rate, the amount of chlorophyll pigments, phicobiliprotein were measured.The amount of total fatty acids, fatty acid profiling were measured by FTIR and GC. Comparison of results between treatments showed the best growth conditions in the concentration of 1.5 (g/L) NaNO3 and pH 7 observed. The greatest amount of pigment and production of fatty acids in a concentration of 0.25 (g/L) NaNO3 and pH 9 was found. The maximum content of lipids were measured such as methyl Myrstat (32.46%), methyl palmitate (16.73%), methyl stearate (5.85%), methyl oleat (1.14%), methyl linoleic (0.64%), methyl linolenic (0%) were measured. Our results indicate that increases lipid production rate in the cyanobacteria Synechococcus elongata in environmental stress conditions.

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