Evaluation of the Effect of Nebivolol in Bone Defect Healing in An Experimental Rat Model

Abstract

Objective: to evaluate the beneficial role of Nebivolol on bone formation in Experimental model of rat. Study Design: Laboratory-based experimental study Place and Duration of Study: Department of Pharmacology, Army Medical College, Rawalpindi Pakistan, from Jun-Aug 2020 Methodology: Fourteen (14) rats of the Sprague Dawley variety were obtained for the study. The bone defect was made in each rat's upper quadrant of the right femur shaft by surgical intervention.1-2mm bone defect was created using a Dental Engine motor, avoiding any injury to muscles or other areas of bone. Rats were divided into two equal groups. The Disease Control Group was labelled as Group-A, which was restricted to normal drinking water and feed for three weeks after surgery. Nebivolol-Group was tagged as Group-B, given Nebivolol (2.5mg/kg) once daily (OD) for three weeks starting postop Day 1. Rats included in the study were sacrificed at the end of the three weeks, and their right femurs were collected in sample jars for radiography and histopathology. Results: The radiographic scores, as well as the histopathology scores, were analyzed. Results depicted noteworthy differences between Group-A and Group-B and found to be highly significant (p-value <0.01). Conclusion: Nebivolol efficaciously enhances bone regeneration in bone defects induced in a rat model.

Keywords

• Anti-oxidant effect
• Bone regrowth,
• Nebivolol
• Nitric oxide