A Comparison Study between Adipose-Derived Stem Cells and Bone Marrow Stromal Cells Based on Self-Renewal Potential of Various Passages

Abstract

Introduction: For many years, bone marrow mesenchymal stem cells (BMSCs) was the main source for tissue engineering, though within recent years adipose tissue-derived stem cells (ADSC) has obtained a special place in this regard, since it seems easier to isolate these cells with fewer complications to the patients. Therefore, the purpose of this study was to compare two main sources of stem cells, ADSCs and BMSCs, considering cell doubling time and the percentage of viable cells during various cell passages. Methods: In this study, with equal Sterile and culture conditions in various cell passages, the percentage of viable cells and cell doubling time were assessed using MTT assay. Moreover, semi quantitative Oct-4 gene expression, RT-PCR method and image j software (1 to 10 passages) were applied to assess proliferation rate. Results: Attained results of this study demonstrated that, at high passages ADSCs have a higher proliferation rate and viable cells compared to BMSCs. Furthermore, semi-quantitative RT-PCR revealed the both cells were able to express Oct-4 and create a distinct band on gel electrophoresis, though ADSCs showed stronger band in comparison with BMSCs. Conclusion: Due to the rapid growth and viability, stem cells deriving from adipose tissue can be considered as a good stem cell source for cell therapy in the clinic in comparison with BMSCs.

Keywords

• ADSCs
• BMSCs
• Cell doubling time
• Proliferation rate