Frontiers in Cell and Developmental Biology (Aug 2023)
Age-dependent structural reorganization of utricular ribbon synapses
- Susann Michanski,
- Susann Michanski,
- Susann Michanski,
- Timo Henneck,
- Mohona Mukhopadhyay,
- Anna M. Steyer,
- Anna M. Steyer,
- Paola Agüi Gonzalez,
- Katharina Grewe,
- Peter Ilgen,
- Peter Ilgen,
- Peter Ilgen,
- Mehmet Gültas,
- Eugenio F. Fornasiero,
- Stefan Jakobs,
- Stefan Jakobs,
- Stefan Jakobs,
- Wiebke Möbius,
- Wiebke Möbius,
- Christian Vogl,
- Christian Vogl,
- Christian Vogl,
- Tina Pangršič,
- Tina Pangršič,
- Tina Pangršič,
- Silvio O. Rizzoli,
- Carolin Wichmann,
- Carolin Wichmann,
- Carolin Wichmann
Affiliations
- Susann Michanski
- Molecular Architecture of Synapses Group, Institute for Auditory Neuroscience, InnerEarLab and Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Göttingen, Germany
- Susann Michanski
- Collaborative Research Center 889, University of Göttingen, Göttingen, Germany
- Susann Michanski
- Multiscale Bioimaging Cluster of Excellence (MBExC), University of Göttingen, Göttingen, Germany
- Timo Henneck
- Biology Bachelor Program, University of Göttingen, Göttingen, Germany
- Mohona Mukhopadhyay
- Experimental Otology Group, InnerEarLab, Department of Otolaryngology, Institute for Auditory Neuroscience, University Medical Center Göttingen, Göttingen, Germany
- Anna M. Steyer
- Electron Microscopy-City Campus, Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
- Anna M. Steyer
- Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), University of Göttingen, Göttingen, Germany
- Paola Agüi Gonzalez
- Department for Neuro-and Sensory Physiology, University Medical Center Göttingen, Center for Biostructural Imaging of Neurodegeneration (BIN), Göttingen, Germany
- Katharina Grewe
- Department for Neuro-and Sensory Physiology, University Medical Center Göttingen, Center for Biostructural Imaging of Neurodegeneration (BIN), Göttingen, Germany
- Peter Ilgen
- Clinic of Neurology, University Medical Center Göttingen, Göttingen, Germany
- Peter Ilgen
- 0Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
- Peter Ilgen
- 1Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Translational Neuroinflammation and Automated Microscopy TNM, Göttingen, Germany
- Mehmet Gültas
- 2Faculty of Agriculture, South Westphalia University of Applied Sciences, Soest, Germany
- Eugenio F. Fornasiero
- Department for Neuro-and Sensory Physiology, University Medical Center Göttingen, Center for Biostructural Imaging of Neurodegeneration (BIN), Göttingen, Germany
- Stefan Jakobs
- Clinic of Neurology, University Medical Center Göttingen, Göttingen, Germany
- Stefan Jakobs
- 0Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
- Stefan Jakobs
- 1Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Translational Neuroinflammation and Automated Microscopy TNM, Göttingen, Germany
- Wiebke Möbius
- Multiscale Bioimaging Cluster of Excellence (MBExC), University of Göttingen, Göttingen, Germany
- Wiebke Möbius
- Electron Microscopy-City Campus, Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
- Christian Vogl
- Collaborative Research Center 889, University of Göttingen, Göttingen, Germany
- Christian Vogl
- 3Presynaptogenesis and Intracellular Transport in Hair Cells Group, Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany
- Christian Vogl
- 4Auditory Neuroscience Group, Institute of Physiology, Medical University Innsbruck, Innsbruck, Austria
- Tina Pangršič
- Collaborative Research Center 889, University of Göttingen, Göttingen, Germany
- Tina Pangršič
- Multiscale Bioimaging Cluster of Excellence (MBExC), University of Göttingen, Göttingen, Germany
- Tina Pangršič
- Experimental Otology Group, InnerEarLab, Department of Otolaryngology, Institute for Auditory Neuroscience, University Medical Center Göttingen, Göttingen, Germany
- Silvio O. Rizzoli
- Department for Neuro-and Sensory Physiology, University Medical Center Göttingen, Center for Biostructural Imaging of Neurodegeneration (BIN), Göttingen, Germany
- Carolin Wichmann
- Molecular Architecture of Synapses Group, Institute for Auditory Neuroscience, InnerEarLab and Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Göttingen, Germany
- Carolin Wichmann
- Collaborative Research Center 889, University of Göttingen, Göttingen, Germany
- Carolin Wichmann
- Multiscale Bioimaging Cluster of Excellence (MBExC), University of Göttingen, Göttingen, Germany
- DOI
- https://doi.org/10.3389/fcell.2023.1178992
- Journal volume & issue
-
Vol. 11
Abstract
In mammals, spatial orientation is synaptically-encoded by sensory hair cells of the vestibular labyrinth. Vestibular hair cells (VHCs) harbor synaptic ribbons at their presynaptic active zones (AZs), which play a critical role in molecular scaffolding and facilitate synaptic release and vesicular replenishment. With advancing age, the prevalence of vestibular deficits increases; yet, the underlying mechanisms are not well understood and the possible accompanying morphological changes in the VHC synapses have not yet been systematically examined. We investigated the effects of maturation and aging on the ultrastructure of the ribbon-type AZs in murine utricles using various electron microscopic techniques and combined them with confocal and super-resolution light microscopy as well as metabolic imaging up to 1 year of age. In older animals, we detected predominantly in type I VHCs the formation of floating ribbon clusters, mostly consisting of newly synthesized ribbon material. Our findings suggest that VHC ribbon-type AZs undergo dramatic structural alterations upon aging.
Keywords
