Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum)

Hirohito Ogawa; Masahiro Kajihara; Naganori Nao; Asako Shigeno; Daisuke Fujikura; Bernard M. Hang’ombe; Aaron S. Mweene; Alisheke Mutemwa; David Squarre; Masao Yamada; Hideaki Higashi; Hirofumi Sawa; Ayato Takada

doi:10.3390/v9120371

Viruses (Dec 2017)

Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum)

Hirohito Ogawa,
Masahiro Kajihara,
Naganori Nao,
Asako Shigeno,
Daisuke Fujikura,
Bernard M. Hang’ombe,
Aaron S. Mweene,
Alisheke Mutemwa,
David Squarre,
Masao Yamada,
Hideaki Higashi,
Hirofumi Sawa,
Ayato Takada

Affiliations

Hirohito Ogawa: Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan
Masahiro Kajihara: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan
Naganori Nao: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan
Asako Shigeno: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan
Daisuke Fujikura: Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan
Bernard M. Hang’ombe: Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
Aaron S. Mweene: Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
Alisheke Mutemwa: Provincial Veterinary Office, Department of Veterinary Services, Ministry of Fisheries and Livestock, P.O. Box 70416, Ndola 50100, Zambia
David Squarre: Department of National Parks and Wildlife, Ministry of Tourism and Arts, Private Bag 1, Chilanga 10101, Zambia
Masao Yamada: Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan
Hideaki Higashi: Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
Hirofumi Sawa: Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
Ayato Takada: Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia

DOI: https://doi.org/10.3390/v9120371
Journal volume & issue: Vol. 9, no. 12
p. 371

Abstract

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Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name “Bat mastadenovirus H”. Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.

Published in Viruses

ISSN: 1999-4915 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/viruses

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