Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8

Abstract

In the present study, the genomes of B. subtilis EM-A7 and B. velezensis EM-A8 were sequenced and annotated. The Illumina sequencing platform (NovaSeq PE150) was used to sequence the genomic DNA. There were 6 277 054 raw reads for EM-A7, with a Q20 of 97.52 % and 43.78 % GC, and 8 030 262 raw reads for EM-A8, with a Q20 of 97.53 % and 46.21 % GC. Annotation was carried out by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). The strains were classified taxonomically on the basis of an average nucleotide identity analysis (ANI), as well as through a dDDh analysis on the Genome-to-Genome Distance Calculator (GGDC v3.0). The pipeline predicted 4062 protein-coding sequences (CDSs) and 73 RNA genes (62 tRNA and 6 rRNA) for EM-A7, and 3797 protein-coding sequences (CDSs) and 80 RNA genes for EM-A8. These findings enhance our understanding of the two strains’ potential as biocontrol agents to manage disease in maize.

Keywords

• Draft genome sequencing
• Biofilm
• Bacillus
• Biocontrol agent
• Phyllosphere