Frontiers in Endocrinology (Jan 2022)
ANGPTL4 Expression in Ovarian Granulosa Cells Is Associated With Polycystic Ovary Syndrome
- Qi Jiang,
- Qi Jiang,
- Qi Jiang,
- Qi Jiang,
- Qi Jiang,
- Ye Pan,
- Ye Pan,
- Ye Pan,
- Ye Pan,
- Ye Pan,
- Ping Li,
- Yanjun Zheng,
- Yanjun Zheng,
- Yanjun Zheng,
- Yanjun Zheng,
- Yanjun Zheng,
- Yuehong Bian,
- Yuehong Bian,
- Yuehong Bian,
- Yuehong Bian,
- Yuehong Bian,
- Wenqi Wang,
- Wenqi Wang,
- Wenqi Wang,
- Wenqi Wang,
- Wenqi Wang,
- Guihua Wu,
- Tian Song,
- Tian Song,
- Tian Song,
- Tian Song,
- Tian Song,
- Yuhua Shi,
- Yuhua Shi
Affiliations
- Qi Jiang
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Qi Jiang
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, China
- Qi Jiang
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Qi Jiang
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Qi Jiang
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China
- Ye Pan
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Ye Pan
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, China
- Ye Pan
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Ye Pan
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Ye Pan
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China
- Ping Li
- Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China
- Yanjun Zheng
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Yanjun Zheng
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, China
- Yanjun Zheng
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Yanjun Zheng
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Yanjun Zheng
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China
- Yuehong Bian
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Yuehong Bian
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, China
- Yuehong Bian
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Yuehong Bian
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Yuehong Bian
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China
- Wenqi Wang
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Wenqi Wang
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, China
- Wenqi Wang
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Wenqi Wang
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Wenqi Wang
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China
- Guihua Wu
- Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China
- Tian Song
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Tian Song
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, China
- Tian Song
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Tian Song
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Tian Song
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China
- Yuhua Shi
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Yuhua Shi
- Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong, China
- DOI
- https://doi.org/10.3389/fendo.2021.799833
- Journal volume & issue
-
Vol. 12
Abstract
ObjectivesThis study aims to characterize the expression of ANGPTL4 in ovarian granulosa cells (GCs) and its association with polycystic ovary syndrome (PCOS).MethodsThis study included 104 PCOS patients and 112 women in control group undergoing in vitro fertilization-embryo transfer (IVF-ET) from the reproductive hospital affiliated with Shandong University from 2019 to 2021. By reverse transcription and real-time quantitative (RT-q) PCR, the mRNA expression of ANGPTL4 in GCs was assessed, and clinical information for these patients were then reviewed and analyzed.ResultsThe RT-qPCR results showed that ANGPTL4 expression in the control group was significantly lower than that in the PCOS group (p = 0.000) and had positive association with AMH (r = 0.211), HOMA-IR (r = 0.174), LDL/HDL (r = 0.176), ApoB/ApoAI (r = 0.155), and TC/HDL (r = 0.189). Additionally, the high expression of ANGPTL4 in the ovarian granulosa cells might be an independent predictor in PCOS (OR: 3.345; 95% CI: 1.951–5.734) with a close contact with incidence of PCOS (AUC: 0.704; 95% CI: 0.633–0.774, p < 0.001).ConclusionsOur study revealed higher ANGPTL4 expression in ovarian GCs with PCOS. Its association with glucose and lipid metabolism showed that ANGPTL4 might play an important role in PCOS metabolism and pathogenesis.
Keywords
- polycystic ovary syndrome
- angiopoietin-like protein 4
- mRNA
- ovarian granulosa cell
- glycolipid metabolism
