Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste

Abstract

Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate (PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB is potential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. The development of PHB production using starch as an alternative carbon source has been conducted to reduce the dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processing site were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of the study showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB ranged from 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 and after optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubation time. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed as Micrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicated as poly-β- hydroxybutyrate (PHB)

Keywords

• Poly-β-hydroxybutyrate (PHB)
• indigenous amylolytic bacteria
• arrowroot starch