Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii

Min Feng; Dezhao Kong; Wenbing Wang; Liqiang Liu; Shanshan Song; Chuanlai Xu

doi:10.3390/s150204291

Sensors (Feb 2015)

Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii

Min Feng,
Dezhao Kong,
Wenbing Wang,
Liqiang Liu,
Shanshan Song,
Chuanlai Xu

Affiliations

Min Feng: Huaian Entry-Exit Inspection and Quarantine Bureau, Huaian 223001, China
Dezhao Kong: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Wenbing Wang: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Liqiang Liu: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Shanshan Song: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Chuanlai Xu: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

DOI: https://doi.org/10.3390/s150204291
Journal volume & issue: Vol. 15, no. 2
pp. 4291 – 4301

Abstract

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A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp. stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 105 cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples.

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

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