An improved two dimensional thin-layer chromatography system for the separation of phosphatidylglycerol and its derivatives

B J H M Poorthuis; P J Yazaki; K Y Hostetler

Journal of Lipid Research (Jul 1976)

An improved two dimensional thin-layer chromatography system for the separation of phosphatidylglycerol and its derivatives

B J H M Poorthuis,
P J Yazaki,
K Y Hostetler

Affiliations

B J H M Poorthuis: Department of Medicine, Division of Metabolic Disease, University of California, San Diego, California; The Veterans Administration Hospital, La Jolla, California 92037
P J Yazaki: Department of Medicine, Division of Metabolic Disease, University of California, San Diego, California; The Veterans Administration Hospital, La Jolla, California 92037
K Y Hostetler: Department of Medicine, Division of Metabolic Disease, University of California, San Diego, California; The Veterans Administration Hospital, La Jolla, California 92037

Journal volume & issue: Vol. 17, no. 4
pp. 433 – 437

Abstract

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A two dimensional thin-layer chromatography system has been devised for the improved separation of phosphatidylglycerol and its derivatives, cardiolipin and bis(monoacylglyceryl)phosphate, from the other phospholipid components of tissue total lipid extracts. The system employs silica gel G plates prepared with 0.4 M boric acid. Linear recovery of added phosphatidylglycerol was found, and phosphatidylglycerol did not cochromatograph with N,N-dimethylphosphatidylethanolamine in this system. The phospholipid class composition of various rat tissues and a Morris 7777 hepatoma has been determined and compared with values from the literature.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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