Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L.

Abstract

The use of in vitro techniques for the production of secondary metabolites in the pharmaceutical industry has increased widely. The organ culture is one of the alternatives for the identification of new compounds or to increase the production of already known compound useful for the humanity. The aim of this work was to produce biomass of Psidium guajava L var ‘EEA18-40’ using shoot culture and to determine the effect of the in vitro culture in the metabolic profile of this spicies. Water content was higher in the plant material obtained in the shoots cultured in vitro compared to samples collected from plants grown in field. Yields of extracts were greater in shoots cultured in vitro. Expression profiles among leaves samples of plants grown in field were different in this species, obtaining a higher rate in the shoots culture.A number of 23 new compounds was detected . This is the first work that shows the metabolic profile of Psidium guajava in shoots cultured in vitro. It was demonstrated that the in vitro culture is a potential source for identification and production of new compounds. Key words: guava, mass spectrometry, organs culture, secondary metabolites